Journal: Reproductive Biology and Endocrinology

Article Title: Progesterone improves the number and quality of hormone induced Fowler toad ( Bufo fowleri ) oocytes

doi: 10.1186/1477-7827-4-3

Figure Lengend Snippet: Toads were randomly placed into seven treatments where hormones were administered alone or in variouscombinations. Treatment 7* was administered two doses of progesterone 12 hrs apart. Then all seven treatments were administered a second dose of compounds either 24 or 48 hrs later and evaluated as a second set of treatments (8–14).

Article Snippet: All the combinations of hormones or Pimozide™ were administered through intra-peritoneal injection in 100 μl of phosphate buffered saline (PBS) (Intervet, Millsboro, De 19966, USA) except for those containing progesterone which were administered in 1 ml of PBS.

Techniques:

Journal: Reproductive Biology and Endocrinology

Article Title: Progesterone improves the number and quality of hormone induced Fowler toad ( Bufo fowleri ) oocytes

doi: 10.1186/1477-7827-4-3

Figure Lengend Snippet: The different doses of hormones or Pimozide™ used in this study and the amountper. gram of toad weight.

Article Snippet: All the combinations of hormones or Pimozide™ were administered through intra-peritoneal injection in 100 μl of phosphate buffered saline (PBS) (Intervet, Millsboro, De 19966, USA) except for those containing progesterone which were administered in 1 ml of PBS.

Techniques:

Journal: Reproductive Biology and Endocrinology

Article Title: Progesterone improves the number and quality of hormone induced Fowler toad ( Bufo fowleri ) oocytes

doi: 10.1186/1477-7827-4-3

Figure Lengend Snippet: Variables measured in response to different hormone treatments including toad length, weight, the number and percentage of spawning toads, the number of oocytes, percent fertilization, number of neurulas, percent neurulation, and total number of neurulas. Treatment 7* was administered two doses of progesterone 12 hrs apart. Data in the table are expressedas Means ± SE and ranges of spawning toads.

Article Snippet: All the combinations of hormones or Pimozide™ were administered through intra-peritoneal injection in 100 μl of phosphate buffered saline (PBS) (Intervet, Millsboro, De 19966, USA) except for those containing progesterone which were administered in 1 ml of PBS.

Techniques:

Journal: Cancers

Article Title: STAT5 is Expressed in CD34 + /CD38 − Stem Cells and Serves as a Potential Molecular Target in Ph-Negative Myeloproliferative Neoplasms

doi: 10.3390/cancers12041021

Figure Lengend Snippet: HEL and SET-2 cells were incubated with various concentrations of JAK2-targeting drugs (ruxolitinib, R763, TG101348, AZD1480) or STAT5-targeting drugs (piceatannol, pimozide, AC-3-019, AC-4-130) for 4 h. Thereafter, expression of pSTAT5 was analyzed by multicolor flow cytometry. The levels of pSTAT5 are depicted as median fluorescence index (MFI) values relative to the untreated control (Co).

Article Snippet: The anti-pSTAT5 alpha (Tyr694) polyclonal Ab was purchased from Invitrogen (order number 71-6900; Carlsbad, CA, USA), anti-pSTAT5 (Y694) Alexa Fluor ® 647 monoclonal Ab (mAb) 47 (order number: 612599) and an isotype-matched control antibody (mIgG1-Alexa Fluor ® 647, order number: 557783) from BD Biosciences Pharmingen (San Jose, CA, USA), piceatannol (order number: P0453) and pimozide (order number: P1793) from Merck (Darmstadt, Germany), and the JAK2 blockers AZD1480 (order number: CT-A1480), TG101348 (order number: CT-TG101) and ruxolitinib (order number: CT-INCB) from ChemieTek (Indianapolis, IN, USA).

Techniques: Incubation, Expressing, Flow Cytometry, Fluorescence, Control

Journal: Cancers

Article Title: STAT5 is Expressed in CD34 + /CD38 − Stem Cells and Serves as a Potential Molecular Target in Ph-Negative Myeloproliferative Neoplasms

doi: 10.3390/cancers12041021

Figure Lengend Snippet: Specifications of inhibitors used in this study.

Article Snippet: The anti-pSTAT5 alpha (Tyr694) polyclonal Ab was purchased from Invitrogen (order number 71-6900; Carlsbad, CA, USA), anti-pSTAT5 (Y694) Alexa Fluor ® 647 monoclonal Ab (mAb) 47 (order number: 612599) and an isotype-matched control antibody (mIgG1-Alexa Fluor ® 647, order number: 557783) from BD Biosciences Pharmingen (San Jose, CA, USA), piceatannol (order number: P0453) and pimozide (order number: P1793) from Merck (Darmstadt, Germany), and the JAK2 blockers AZD1480 (order number: CT-A1480), TG101348 (order number: CT-TG101) and ruxolitinib (order number: CT-INCB) from ChemieTek (Indianapolis, IN, USA).

Techniques: Inhibition, Concentration Assay

Journal: Cancers

Article Title: STAT5 is Expressed in CD34 + /CD38 − Stem Cells and Serves as a Potential Molecular Target in Ph-Negative Myeloproliferative Neoplasms

doi: 10.3390/cancers12041021

Figure Lengend Snippet: IC 50 concentrations of JAK2- and STAT5 targeting drugs in pSTAT5 high and low cell lines.

Article Snippet: The anti-pSTAT5 alpha (Tyr694) polyclonal Ab was purchased from Invitrogen (order number 71-6900; Carlsbad, CA, USA), anti-pSTAT5 (Y694) Alexa Fluor ® 647 monoclonal Ab (mAb) 47 (order number: 612599) and an isotype-matched control antibody (mIgG1-Alexa Fluor ® 647, order number: 557783) from BD Biosciences Pharmingen (San Jose, CA, USA), piceatannol (order number: P0453) and pimozide (order number: P1793) from Merck (Darmstadt, Germany), and the JAK2 blockers AZD1480 (order number: CT-A1480), TG101348 (order number: CT-TG101) and ruxolitinib (order number: CT-INCB) from ChemieTek (Indianapolis, IN, USA).

Techniques: